Single Cell Technologies Trends 2014
|出版日||ページ情報||英文 60 Pages
This market report summarizes the results of HTStec's 2nd industry-wide global web-based benchmarking survey on single cell technologies carried out in September 2014.
The survey was initiated by HTStec as part of its tracking of emerging life science marketplaces and to update our earlier report (dated July 2012).
The objectives were to understand current interest and requirements for single cell technologies.
The questionnaire was compiled to meet the needs and interests of our clients, who were manufacturers/developers of laboratory equipment, reagents and consumables for the separation and analysis of single cells. They seek to gain insight in the application requirements, market opportunities, unmet needs and demand for single cell technology-related products.
Equal emphasis was given to soliciting opinion from all organizations interested in single cell separation or single cell analysis technologies with no geographical bias in the persons contacted.
The survey looked at the following aspects of single cell technologies, as practiced today (2014) and in a few cases as predicted for the future (2016): current use of single cells or single cell enabling technologies; market segments that best match respondents use and categorization of single technologies; scientific research area where interest in single cell technologies best fits; importance placed on the use/application of single cell technologies; main reasons for using a single cell versus an average of a pooled population of many cells; the value of the specific information derived by single cell technologies and whether it can be directly compared to pooled population of many cells; current use of technologies to separate single cells; analysis technologies applied to single cells; are available instrumentation for downstream (post-separation) single cell analysis fit for purpose; cell types used for single cell applications; main sources of isolated single cells; level of purity acceptable in isolated single cells; intended downstream work on isolated single cells; main applications of single cells and where single cells will impact most in the future; functions of a single cell enabling technology of greatest importance to respondent's research; number of single cells needed per experiment and frequency of running single cell experiments; destination substrate or vessel for isolated single cell delivery and transfer liquid volume per cell; main limitations of currently available single cell separation and analysis technologies; likelihood of purchasing any new instrumentation to enable single cell separation or single cell analysis; capex and consumable budgets for single cell separation or single cell analysis; awareness of key players (vendors) in the field of single cell separation or single cell analysis technologies; resources used to learn about new single cell technologies; conferences attended in the past 12 months in the pursuit of single cell technology information gathering/debate; publications respondents subscribe to, regularly read or visit online; and any improvements respondents require or unmet needs in single cell technologies.
The main questionnaire consisted of 29 multi-choice questions and 1 open-ended question. In addition, there were 7 questions related solely to survey demographics.
The survey collected 147 validated responses, of these 76% provided comprehensive input.
Survey responses were geographically split: 43% Europe; 41% North America; 10% Asia (excluding Japan & China); 3% Japan; 3% Rest of World; and 1% China.
Survey respondents were drawn from persons or groups currently using or planning future use of single cells or single cell enabling technologies in their research efforts.
Respondents came from 90 University/Research Institute/Not-for-Profit Facilities; 20 Biotechs; 12 Pharma; 7 Diagnostics; 6 Hospitals/Clinics; 4 Government/Military/Defence Facilities; 3 Contract Research Organisations; 3 Other; 1 Chemicals; and 1 Agri Biotech/Plant Genomics.
Most survey respondents had a senior job role or position which was in descending order: 27 professors/ assistant professors; 23 research scientists; 18 principal investigators; 16 senior scientists/research associates; 16 other roles; 13 post-docs; 10 directors; 9 section/group leaders; 6 department heads; 4 graduate students; 2 lab/research managers; and 2 vice presidents.
Survey results were expressed as an average of all survey respondents. In addition, where appropriate the data was fully reanalyzed after sub-division into 6 survey groups: 1) Basic Research; 2) Applied Research; 3) Pharma Research; 4) Clinical Research; 5) Primarily Genomic; & 6) Primarily Proteomic.
64% of respondents were currently using single cells or single cell technologies in their research.
Respondents were segmented on the basis of their current field of interest and use of single cell technologies: 44% basic research, 33% applied research; 12% clinical research; & 10% pharma research.
Respondent's interest can be further categorized as 36% primarily genomic and 13% primarily proteomic.
The scientific area where respondent's interest in single cell technologies most fits was oncology/cancer.
Most respondents placed high importance on the use of single cell technologies today.
Reveals intracellular differences in a heterogeneous population was ranked the main reason for using a single cell versus an average of a pooled population of many cells.
Most respondents rate the value of specific information derived from single cell technologies highly versus the average of a pooled population of many cells.
Most respondents thought information derived by single cell technologies can be compared to an average of a pooled population of many cells, but it is unlikely they will show >50% complementary.
FACS/flow cytometry was rated as the technology currently most used to isolate single cells from tissues or liquid culture today.
The analysis technologies most applied to single cells were fluorescence microscopy, FACS/flow cytometry and brightfield microscopy.
Of available instrumentation for single cell analysis fluorescence microscopy was rated as most adequate.
The most used cell type for single cell technology applications was primary cells.
The source most used to isolate single cells from was adherent cell cultures.
The median levels of acceptable purity in isolated single cells were as follows: desired cell phenotype >99.5%; cells that are singlet (not clumped) >99.5%; cells free from attached debris >99%; viable cells >95%; and cell cycle stage-specific >95%.
Most downstream work on isolated cells involves their immediate destruction to extract/purify cellular components for analysis.
Gene expression profiling was the application most applied/investigated with single cell technologies.
Biomarker discovery, validation and/or screening were the application expected to be impacted most by single cell technologies over the coming years.
The ability to pick a single cell with a specific phenotype from a heterogeneous cell mixture was rated as the function of a single cell enabling technology of greatest importance to respondent's research.
The median current or potential use of single cells were as follows: 100 isolated single cells per experiment; approximately 1 single cell experiment per week; main destination substrate/vessel for isolated single cells - 96-well plate; and transfer liquid volume per cell - 100nL-1uL.
High cost of equipment was rated the most limiting aspect of single cell separation technologies.
High cost per experiment was rated the most limiting aspect of single cell analysis technologies.
The likelihood respondents will purchase any new instruments for single cell separation or single cell analysis over the next few years (up to 2016) was possible (26-50% probability).
The median actual 2013 annual capex (instrument) budgets were $10K-$25K for single cell separation technologies and $10K-$25K for single cell analysis technologies.
The median actual annual consumable budgets spent in 2013 were $5K-$10K for single cell separation technologies and $5K-$10K for single cell analysis technologies.
Several bottom-up models were developed around the respondent's budgets to calculate the global single cell technologies markets. In 2014 these were estimated for single cell separation to be around $126M for instruments and $78M for consumables. In 2014 these were estimated for single cell analysis to be around $98M for instruments and $83M for consumables. Segmentation & CAGR estimates are given in the full report.
The top 3 vendors for single cell separation were Becton Dickenson, Fluidigm and Miltenyi Biotec.
The top 3 vendors for single cell analysis were Fluidigm, Life Technologies and Becton Dickenson.
Peer-reviewed publications were the resource most used to learn about new single cell technologies.
Most respondents have not attended any conferences in the last 12 months in the pursuit of new single cell technology information/debate.
The publications respondents subscribe to, regularly read or visit online most were Nature and Science.
Feedback on improvements required and unmet needs in single cell technologies were documented. One theme to emerge was the lack of a clear vision on the potential impact/value/applicability of single cell research.
The full report provides the data, details of the breakdown of the responses for each question, its segmentation and some estimates for the future (2016). It also highlights some interesting differences between the survey groups.