核酸分離：シリーズ1

Nucleic Acid Separation Dashboard: Series One

発行	Percepta Associates Inc.
出版日	2008年11月	商品コード	78477
ページ情報	英文
価格	US$ 3,500 換算 ¥ 281,645 (税抜) Company Wide PDF Price

概要
原文目次
関連レポート

原文目次

Abstract

Overview

The separation of nucleic acids is a necessary precursor to a wide range of life science research and diagnostic techniques. Familiar methods such as cloning/subcloning and Southern and Northern blotting frequently rely on the electrophoretic separation of nucleic acid fragments generated by amplification or restriction digestion. Recently emerging methods such as gene silencing and miRNA analyses often hinge on the separation and sizing of short polynucleotides isolated from cell or tissue samples or synthesized in vitro. For some techniques, such as mutation detection or STR analysis, resolution in the range of a few bases may be required. For other methods, like standard cloning or Southern blotting, a resolution in the tens to hundreds of bases may suffice.

Percepta' s 2008 Nucleic Acid Separation Dashboard™ dives deeply into the characteristics of the market for nucleic acid separation products. This Dashboard reveals key market indicators for the nucleic acids separations market as a whole as well as for the following sub-segments:

Analysis of amplified DNA (includes AFLP) Analysis of restriction digested DNA (includes RFLP)
Analysis of cRNA or total RNA quality
Analysis of small RNA molecules Analysis of synthetic DNA or RNA
STR analysis
HLA typing analysis
Pulsed field gel electrophoresis (includes mapping)
Mutation detection (includes SSCP, DGGE)

Survey Methodology

In August of 2008, Percepta fielded the 23-question Nucleic Acid Separation Survey to a subset of the company' s panel of life scientists. Individuals were invited by e-mail to click through to a webpage at bioanalytix.com where the survey was hosted. Invitations were delivered on August 19, 2008 and results collected through August 26th. A total of 548 scientists completed the survey, of which 526 are actively engaged in performing nucleic acid separation and 8 plan to perform nucleic acid separation in the next 12 months. Results based on the aggregate of collected responses are revealed in this Nucleic Acid Separation Life Science Dashboard™.

Respondent Demographics

Respondents from the academic, government and commercial market segments are well represented, with 13.6% of respondents employed in an industry setting. 72.5% of respondents are from North America, while 24.8% reside in Europe.

Junior (Lab Tech, Grad Students), mid level (Post-Doc, Lab Manager) and senior (Professor/PI, Group Leader) scientists are well represented in the data set, with the most cited job titles being

Professor/Principal Investigator (19.2% of respondents), Scientist/Senior Scientist (17.3% of respondents) and Post-Doctoral Fellow (14.6%).

A wide variety of scientific areas of specialization is also evident, led by Molecular Biology (named by 34.9% of respondents as their primary area of expertise) and Microbiology/Infectious Disease/Virology (named by 9.9% of respondents). Biochemistry (9.7%), Genetics (8.6%), and Cell Biology (7.3%) are the only other primary areas of specialization named by more than 6% of respondents.

Small (1-5 scientists), medium (6-20 scientists) and large (>20 scientists) laboratories are well represented: 39.6% of respondents work in labs where 1 to 5 people perform experiments; 47.1% in labs with 6 to 20, and the remaining 13.3% in labs with greater than 20 bench scientists.

Figures and Tables
Executive Summary
Key Findings and Implications
Nucleic Acid Separation Dashboard
Survey Methodology
Survey Invitation Text
Respondent Demographics
Frequency of Performance of Life Science Techniques
Frequency of Performance of Various Nucleic Acid Separation Techniques
Reaction Throughput and Market Segment Growth Rates
Respondent' s Stated Price Per Reaction
Total Market Size, Market Segment Sizes and Total Market Growth Rate
Matrix/Method Used to Separate Various Types of Nucleic Acids
Market Shares by Segment (Share of Mention)
Required Resolution for Nucleic Acid Separation Experiments
Required Throughput for Nucleic Acid Separation Experiments
Time Required for Nucleic Acid Separation Experiments
Customer Satisfaction and Interest in Switching Suppliers
Product Features that Influence Purchasing Decisions
Desired Changes to Nucleic Acid Separation Products
Survey Questionnaire

Figures and Tables

Figure 1: Respondent' s Place of Employment
Figure 2: Respondent' s Country/Region
Figure 3: Respondent' s Position/Job Title
Figure 4A: Respondent' s Areas of Expertise/Specialization
Figure 4B: Respondent' s Areas of Expertise/Specialization (Molecular Biology Excluded)
Figure 5: Number of Employees in Respondent' s Laboratories
Figure 6: Percentage of Respondents Performing Various Life Science Techniques at Least a Few Times per Year
Figure 7: Percentage of Respondents Performing Nucleic Acid Separation
Figure 8: Percentage of Respondents Performing Various Nucleic Acid Separation Methods at Least a Few Times per Year
Figure 9: Percentage of Respondents that Analyze Amplified DNA (Includes AFLP)
Figure 10: Percentage of Respondents that Analyze Restriction Digested DNA (Includes RFLP)
Figure 11: Percentage of Respondents that Analyze cRNA or Total RNA Quality
Figure 12: Percentage of Respondents that Analyze Small RNA Molecules
Figure 13: Percentage of Respondents that Analyze Synthetic DNA or RNA
Figure 14: Percentage of Respondents that Perform STR Analysis
Figure 15: Percentage of Respondents that Perform HLA Typing Analysis
Figure 16: Percentage of Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
Figure 17: Percentage of Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
Figure 18: Method/Matrix Used by Respondents that Analyze Amplified DNA (Includes AFLP)
Figure 19: Method/Matrix Used by Respondents that Analyze Restriction Digested DNA7 (Includes RFLP)
Figure 20: Method/Matrix Used by Respondents that Analyze cRNA or Total RNA Quality
Figure 21: Method/Matrix Used by Respondents that Analyze Small RNA Molecules
Figure 22: Method/Matrix Used by Respondents that Analyze Synthetic DNA or RNA
Figure 23: Method/Matrix Used by Respondents that Perform STR Analysis
Figure 24: Method/Matrix Used by Respondents that Perform HLA Typing Analysis
Figure 25: Method/Matrix Used by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
Figure 26: Method/Matrix Used by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
Figure 27: Respondent' s Primary Supplier of Consumables for Pour-Your-Own Agarose Gels
Figure 28: Respondent' s Primary Supplier of Instruments Used to Run Pour-Your-Own Agarose Gels
Figure 29: Respondent' s Primary Supplier of Precast Agarose Gels
Figure 30: Respondent' s Primary Supplier of Instruments Used to Run Precast Agarose Gels
Figure 31: Respondent' s Primary Supplier of Consumables for Separation of Nucleic Acids by Capillary Electrophoresis
Figure 32: Respondent' s Primary Supplier of Capillary Electrophoresis Instrumentation for Separation of Nucleic Acids
Figure 33: Respondent' s Primary Supplier of Consumables for Microfluidics-Based Separation of Nucleic Acids
Figure 34: Respondent' s Primary Supplier of Instrumentation for Microfluidics-Based Separation of Nucleic Acids
Figure 35: Respondent' s Primary Supplier of Consumables Used to Run Polyacrylamide Gels
Figure 36: Respondent' s Primary Supplier of Instruments Used to Run Polyacrylamide Gels
Figure 37: Resolution Required by Respondents that Analyze Amplified DNA (Includes AFLP)
Figure 38: Resolution Required by Respondents that Analyze Restriction Digested DNA (Includes RFLP)
Figure 39: Resolution Required by Respondents that Analyze cRNA or Total RNA Quality
Figure 40: Resolution Required by Respondents that Analyze Small RNA Molecules
Figure 41: Resolution Required by Respondents that Analyze Synthetic DNA or RNA
Figure 42: Resolution Required by Respondents that Perform STR Analysis
Figure 43: Resolution Required by Respondents that Perform HLA Typing Analysis
Figure 44: Resolution Required by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
Figure 45: Resolution Required by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
Figure 46: Separation Throughput Required by Respondents that Analyze Amplified DNA (Includes AFLP)
Figure 47: Separation Throughput Required by Respondents that Analyze Restriction Digested DNA (Includes RFLP)
Figure 48: Separation Throughput Required by Respondents that Analyze cRNA or Total RNA Quality
Figure 49: Separation Throughput Required by Respondents that Analyze Small RNA Molecules
Figure 50: Separation Throughput Required by Respondents that Analyze Synthetic DNA or RNA
Figure 51: Separation Throughput Required by Respondents that Perform STR Analysis
Figure 52: Separation Throughput Required by Respondents that Perform HLA Typing Analysis
Figure 53: Separation Throughput Required by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
Figure 54: Separation Throughput Required by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
Figure 55: Completion Time Required by Respondents that Analyze Amplified DNA (Includes AFLP)
Figure 56: Completion Time Required by Respondents that Analyze Restriction Digested DNA (Includes RFLP)
Figure 57: Completion Time Required by Respondents that Analyze cRNA or Total RNA Quality
Figure 58: Completion Time Required by Respondents that Analyze Small RNA Molecules
Figure 59: Completion Time Required by Respondents that Analyze Synthetic DNA or RNA
Figure 60: Completion Time Required by Respondents that Perform STR Analysis
Figure 61: Completion Time Required by Respondents that Perform HLA Typing Analysis
Figure 62: Completion Time Required by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
Figure 63: Completion Time Required by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
Figure 64: Percentage of Respondents That Have Switched Suppliers in the Last Six Months
Figure 65: Most Important Features of Products for Nucleic Acid Separation Experiments

Table 1: Respondent' s Areas of Expertise/Specialization - Values for Figures 4A and 4B
Table 2: Frequency of Performance of Various Life Science Techniques
Table 3: Frequency of Co-Performance of Various Life Science Techniques
Table 4: Frequency of Performance of Nucleic Acid Separation Methods
Table 5: Frequency of Co-Performance of Life Science Techniques with Nucleic Acid Separation Methods
Table 6: Frequency of Co-Performance of Nucleic Acid Separation Methods with Life Science Techniques
Table 7: Median and Average Monthly Throughput for Nucleic Acid Separation Techniques
Table 8: Percentage of Respondents Separating Various Numbers of Nucleic Acid Samples (Lanes) Per Month
Table 9: Projected Growth in the Performance of Various Nucleic Acid Separation Techniques
Table 10: Median and Average Price Per Sample for Nucleic Acid Separation Products
Table 11: Estimated 2008 Global Market Size for Nucleic Acid Separation Product Categories
Table 12: Estimated 2009 Global Market Size for Nucleic Acid Separation Product Categories
Table 13: Matrix/Method Used to Separate Various Types of Nucleic Acids
Table 14: Market Share Leaders for Consumables for Pour-Your-Own Agarose Gels by Market Segment
Table 15: Market Share Leaders for Instruments for Pour-Your-Own Agarose Gels by Market Segment
Table 16: Market Share Leaders for Consumables for Precast Agarose Gels by Market Segment
Table 17: Market Share Leaders for Instruments for Precast Agarose Gels by Market Segment
Table 18: Market Share Leaders for Consumables for Capillary Electrophoresis by Market Segment
Table 19: Market Share Leaders for Instruments for Capillary Electrophoresis by Market Segment
Table 20: Market Share Leaders for Consumables for Microfluidics-Based Separation of Nucleic Acids by Market Segment
Table 21: Market Share Leaders for Instruments for Microfluidics-Based Separation of Nucleic Acids by Market Segment
Table 22: Market Share Leaders for Consumables for Polyacrylamide Gels by Market Segment
Table 23: Market Share Leaders for Instruments for Polyacrylamide Gels by Market Segment
Table 24: Market Share Leaders for Nucleic Acid Separation Products
Table 25: Resolution Required for Separation of Various Nucleic Acid Separation Methods
Table 26: Throughput Required for Various Nucleic Acid Separation Methods
Table 27: Completion Time Required for Various Nucleic Acid Separation Methods
Table 28: Percentage of Respondents Satisfied with Various Nucleic Acid Separation Products and Reasons for Dissatisfac tion
Table 29: Most Important Features of Products for Nucleic Acid Separation Experiments