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タンパク質の精製と回収 1月9日〜10日 「効果が実証済みの」手法を用いたタンパク質精製技術の刷新というテーマが基調となるこの部会では、生産性の高い細胞培養技術によって生み出された各種の要求に対応するための取り組みを進めるなかで得られた経験などを専門家たちが紹介するケーススタディなどが予定されています。また、アフィニティータグ、電気泳動法、遠心分離、ろ過、クロマトグラフィー、オペレーショナルエクセレンスプログラムの導入によるプロセスの効率化などに関する詳細なプレゼンテーションを通じて精製プロセスを最適化するための方法が明らかにされるほか、従来型の哺乳類系に加え、大腸菌やバキュロウィルス、酵母などの「特殊な」系を含めた各種の発現系に対応する精製技術も紹介されることになっています。 クロマトグラフィー Capture and Resolution of mAb Charge Variants with Cation Exchangers - Advances and Perspectives Giorgio Carta, Ph.D., Lawrence R. Quarles Professor, Department of Chemical Engineering, University of Virginia Multi-Dimensional Chromatography for Monitoring Quality Attributes of Monoclonal Antibodies in Cell Culture Process Methal Albarghouthi, Ph.D., Senior Scientist, Analytical Biochemistry, MedImmune, LLC
親和性精製 Ultra Pure Functional Protein Recovery with a Novel High Affinity EF Hand Fusion Protein System David O’Connell, Ph.D., Senior Scientist, School of Medicine, University College Dublin Quantification of a PEGylated Peptide Drug Candidate in Human Plasma Using Immuno-Affinity Purification with an Anti-PEG Antibody Followed by LC/LC-MS/MS John T. Mehl, Ph.D., Senior Research Chemist, Vaccine Bioprocess Research & Development, Merck Research Laboratories
プロセスの刷新 Adaptation of Precipitation Technologies for Improved Downstream Efficiency for Monoclonal Antibody Production Alahari Arunakumari, Ph.D., President, AHA Bioconsultants LLC One-Second Isolation/Immunoprecipitation of Weak Protein Complexes Using a Microfluidic IFAST Device Richard R. Burgess, Ph.D., Professor, Oncology, McArlde Lab for Cancer Research, School of Medicine, University of Wisconsin, Madison Process Development of Primary Recovery Steps for a High Cell Density Product Stream Jessica Molek, Ph.D., Principal Scientist, Downstream Process Development, GlaxoSmithKline Rhobust® Technology – Process Intensification Through Direct Product Capture Spencer Reynolds, Director, Business Development, DSM Biologics
新たなタンパク質と精製の困難なタンパク質 Differential Filtration Analysis: An Enabling Technology for Membrane Proteins (and Soluble Proteins too!) Michael C. Wiener, Ph.D., Associate Professor, Molecular Physiology and Biological Physics, Center for Membrane Biology, University of Virginia
Expression and Purification of Novel Viral Fusion Proteins for Drug Target Discovery David Bienvenue, Ph.D., Associate Director, VLST Corporation Protease-Based Nanomachines Philip N. Bryan, Professor, Institute for Bioscience and Biotechnology Research, and Department of Bioengineering, University of Maryland タグ技術 Expression and in vivo Ulp1-Mediated Cleavage of SUMO Fusion Proteins Albert J. Courey, Ph.D., Professor, Chemistry & Biochemistry, UCLA The CK-Tag: A Novel Solution for Protein Expression and Purification David Smith, Ph.D., Senior Scientist, Nuffield Department of Clinical Laboratory Sciences, University of Oxford
精製中のタンパク質の状態監視 Analysis of Protein Samples by Microfluidics: the Pros and Cons Sabine Suppmann, Ph.D., Protein Expression and Purification Core Facility, Max Planck Institute of Biochemistry |
