下流での成功につながる上流での判断

1月9日～10日

生物学的療法に利用可能な高品質のタンパク質に対する需要がかつてない規模に拡大しています。機能性タンパク質の生産をこれまでよりも高い水準に引き上げるという目標を達成するには、工学（科学）と洞察（方法論）が必要になります。生物療法用タンパク質の効果的な発現という課題は、科学と方法論の両方に関わるテーマなのです。この部会は、生物療法用の機能性タンパク質製品へとつながるようなタンパク質発見研究にタンパク質工学の効果的な戦略を応用するという前回までのテーマを踏襲したもので、経験豊富な研究者が披露する現場での経験や応用技術、研究成果などから多くのことを学び取ることができます。

Efficient Optimization of Protein Expression Using High-Throughput Gene Synthesis and Screens

Jingdong Tian, Ph.D., Assistant Professor, Biomedical Engineering, Duke University

Semi-Automatic Cloning and Expression of Immunoglobulin Genes from Single-Plasma Cells for High-Throughput Generation of Monoclonal Antibodies

Masaharu Isobe, Ph.D., Professor, Molecular and Cellular Biology, Life Sciences and Bioengineering, University of Toyama

The Use of a Combinatorial Recombinational Cloning Platform to Simplify Construction of Expression Vectors for Wide-Ranging Areas of Proteomic and Genetic Research

Dominic Esposito, Ph.D., Interim Director, Protein Expression Laboratory; Group Leader, Clone Optimization Group, Advanced Technology Program, SAIC-Frederick, Inc.

Parallel Construct Expression Evaluation Using a High-Throughput Process for Baculovirus-Infected Insect Cells

W. Clay Brown, Ph.D., High-Throughput Protein Lab, Center for Structural Biology, Life Sciences Institute, University
of Michigan

Re-Engineering Multicloning Sites for Function and Convenience

Hal Alper, Ph.D., Assistant Professor, Chemical Engineering, University of Texas at Austin

So Many Options, So Little Money: How to Choose the Right Vector and Screen for Functional Proteins

Bill Gillette, Ph.D., Senior Scientist, Group Leader, Protein Expression Laboratory, Advanced Technology Program, SAIC-Frederick, Inc.

Maximizing Recombinant Protein Expression Through Systematic Gene Design

Mark Welch, Director, Gene Design, DNA2.0

Engineering and Evaluation of Mammalian Secreted Protein Expression Library for High-Throughput Target Discovery

Tadas Panavas, Ph.D., Senior Scientist, Molecular and Protein Biosciences, Centocor R&D, Inc.

Development of Expression-Ready Constructs for Generation of Proteomic Libraries

Susan Celniker, Ph.D., Co-Director, Life Sciences, Lawrence Berkeley National Laboratory

Driving a Variant of the Methanol-Inducible AOX1 Promoter from Pichia pastoris with Glycerol for High-Level Expression of (Therapeutic) Proteins

Roland Weis, Ph.D., Head, Operations, VTU Technology GmbH, Austria

Influence of Histidine Affinity Tags on Protein Structural Order

Evelina Angov, M.S., Ph.D., Microbiologist, Malaria Vaccine Development, Walter Reed Army Institute of Research